G4142

β-Galactosidase from bovine testes

ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)

• Synonym(s): β-D-Galactoside galactohydrolase
• CAS Number: 9031-11-2
• Enzyme Commission number: 3.2.1.23 (BRENDA, IUBMB)
• EC Number: 232-864-1
• MDL number: MFCD00130623
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine
• form: ammonium sulfate suspension
• specific activity: 1.0-3.0 units/mg protein (modified Warburg-Christian)
• foreign activity: α- and β-glucosidase ≤0.2%; α-fucosidase ≤0.2%; α-galactosidase ≤0.2%; α-mannosidase ≤0.2%; β-N-acetylglucosaminidase ≤1.0%
• storage temp.: 2-8°C

Description

Application

β-Galactosidase from bovine testes was used for coupling to Sepharose.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 5.0

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves