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F9512

Sigma-Aldrich

Anti-Human IgG (Fc specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat, Anti Human Igg Fitc

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About This Item

MDL number:
MFCD00162438
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG.

Specificity

Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples

Immunogen

Purified human IgG Fc fragment

Application

Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in:
  • immunofluorescence
  • immunohistochemistry
  • surface labeling experiments

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense.

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reevaluation of flow cytometry for investigating antibody binding to the surface of Plasmodium falciparum trophozoite-infected red blood cells
Williams TN and Newbold CI
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 56(2), 96-103 (2003)
The Immunoglobulins: Structure and Function (1998)
Tryptase is a candidate autoantigen in rheumatoid arthritis
Guo Y, et al.
Immunology, 142(1), 67-77 (2014)
T N Williams et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 56(2), 96-103 (2003-11-11)
The acquisition of antibodies directed toward variant surface antigens (VSAs) expressed on the surface of the trophozoite-infected red blood cell is an important determinant of natural immunity to Plasmodium falciparum malaria. In recent years, flow cytometry has been used increasingly
Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-kappaB signaling pathway
Wang L, et al.
Oncology Letters, 12(6), 4411-4418 (2016)
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