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Key Documents

W3/25

84112002, Reactivity: Anti-CD4, rat

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS1/1

Reactivity

Anti-CD4, rat

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against surface antigens found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages. The antigen has a molecular weight of approximately 50kDa. W3/25 positive T cells have been identified as T helper cells.

Immunogen

Rat thymocyte membrane

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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