LIM2405 cell line human
12062003
Synonym(s):
LIM 2405, LIM-2405
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About This Item
UNSPSC Code:
41106514
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biological source
human colon
growth mode
adherent
morphology
epithelial
shipped in
dry ice
storage temp.
−196°C
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Cell Line Description
Human adenocarcinoma of the caecum of a male patient. Cell Bank Australia reference number: CBA0165.
Culture Medium
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM
Subculture Routine
Optimal split ratio 1:4-1:6 (seeding density 1.6 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM2405 may take 2-3 days to seed after thawing or trypsinisation.
Other Notes
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Certificates of Analysis (COA)
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