B8563
Aniline Blue solution
2.5% in 2% acetic acid
Synonym(s):
Acid blue 93; Cl 42780
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About This Item
Empirical Formula (Hill Notation):
C32H25N3O9S3Na2
CAS Number:
Molecular Weight:
737.73
UNSPSC Code:
41116121
NACRES:
NA.47
Recommended Products
form
liquid
Quality Level
concentration
2.5% in 2% acetic acid
technique(s)
microbe id | staining: suitable
color
dark blue
solubility
water: soluble
λmax
0.21-0.37 at 597-603 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
InChI
1S/C32H27N3/c1-23-22-26(16-21-31(23)33)32(24-12-17-29(18-13-24)34-27-8-4-2-5-9-27)25-14-19-30(20-15-25)35-28-10-6-3-7-11-28/h2-22,34H,33H2,1H3
InChI key
LBLFVSANTXZPEX-UHFFFAOYSA-N
General description
Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.
Application
Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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