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A7979

Sigma-Aldrich

Bovine Serum Albumin solution

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.75

biological source

bovine

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

Assay

33-37% total protein basis (UV)

form

liquid

origin

USA origin

concentration

35% in DPBS

technique(s)

blocking: suitable
cell culture | mammalian: suitable

impurities

≤1000 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile On raw material, none detected

shipped in

ambient

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Select the right albumin for your application with the Albumin Selection Guide.
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neil T Burford et al.
Assay and drug development technologies, 15(7), 320-329 (2017-11-10)
Homogeneous functional assays that utilize competition binding technology are widely used for determining pharmacological properties such as intrinsic activity and potency. One example is time-resolved fluorescence resonance energy transfer (TR-FRET) 3',5'-cyclic adenosine monophosphate (cAMP) assays, where labeled cAMP (tracer) and
Gustavo A Gomez et al.
Developmental biology, 449(2), 99-106 (2019-03-04)
The developmental biology of neural crest cells in humans remains unexplored due to technical and ethical challenges. The use of pluripotent stem cells to model human neural crest development has gained momentum. We recently introduced a rapid chemically defined approach
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
John P Ray et al.
Nature communications, 11(1), 1237-1237 (2020-03-08)
Genome-wide association studies have associated thousands of genetic variants with complex traits and diseases, but pinpointing the causal variant(s) among those in tight linkage disequilibrium with each associated variant remains a major challenge. Here, we use seven experimental assays to
Bryne Ulmschneider et al.
The Journal of cell biology, 215(3), 345-355 (2016-11-09)
Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle
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Articles

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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