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85L1

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-MX phosphate and fast blue RR salt

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.47

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-MX phosphate alkaline solution with fast blue RR salt. The resulting blue, insoluble, granular dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Also Available Separately

Product No.
Description
SDS

  • MHS1Mayer′s Hematoxylin Solution 100 mLSDS

  • 855Naphthol AS-MX Phosphate Alkaline Solution, 0.25% 20 mLSDS

  • FBS25Fast Blue RR Salt, Preweighed Capsules 10 capsulesSDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alban J Smith et al.
Scientific reports, 7, 41872-41872 (2017-02-06)
The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling.
Lee W Tien et al.
Macromolecular bioscience, 12(12), 1671-1679 (2012-10-17)
The alignment and osteogenic differentiation of MSCs on patterned silk films (PF) is investigated as a bottom-up approach toward engineering bone lamellae. Screening PF with various groove dimensions shows that cell alignment is mediated by both the pattern width and
Shigeki Ohta et al.
PloS one, 6(1), e16182-e16182 (2011-01-21)
Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation
Jonathan Ferrand et al.
PloS one, 6(12), e29007-e29007 (2012-01-05)
The role of bone marrow-derived mesenchymal stem cells (MSC) in the physiology of the gastrointestinal tract epithelium is currently not well established. These cells can be recruited in response to inflammation due to epithelial damage, home, and participate in tissue
Masaharu Hiratsuka et al.
PloS one, 6(10), e25961-e25961 (2011-10-15)
Human artificial chromosomes (HACs) have unique characteristics as gene-delivery vectors, including episomal transmission and transfer of multiple, large transgenes. Here, we demonstrate the advantages of HAC vectors for reprogramming mouse embryonic fibroblasts (MEFs) into induced pluripotent stem (iPS) cells. Two

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