12122002

REM 134 Cell Line canine

• UNSPSC Code: 41106514

Properties

• biological source: canine mammary glands
• Quality Level: 100
• growth mode: adherent
• morphology: mixed population of polygonal cells
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

REM 134 is a canine mammary carcinoma cell line derived from a spontaneous primary tumour.

Culture Medium

MEM (M2279) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: REM 134 (ECACC 12122002)

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable