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62087

Millipore

Levine EMB Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Eosin methylene Blue Lactose Agar according to Levine, Levine’s Eosin Methylene Blue Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
dipotassium hydrogen phosphate, 2.0 g/L
eosin yellowish, 0.4 g/L
lactose, 10.0 g/L
meat peptone, 10.0 g/L
methylene blue, 0.065 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
clinical testing
environmental
food and beverages
water monitoring

microbiology

suitability

selective and differential for Candida spp.
selective and differential for Enterobacter spp.
selective and differential for Enterococcus spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for Proteus spp.
selective and differential for Streptococcus spp.
selective and differential for coliforms

Application

Isolation and differentiation of E. coli and Enterobacter, and identification of Candida albicans according to Levine.

Preparation Note

Dissolve 37.5 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Other Notes

Isolation and identification of coagulase-positive staphylococci; Screening method to isolate E. coli from ground beef

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A.J.G. Okrend et al.
Journal of Food Protection, 53, 249-249 (1990)
Occurrence of verocytotoxin-producingEscherichia coli O157 on Dutch dairy farms.
Heuvelink, A. E., et al.
Journal of Clinical Microbiology, 36 (12), 3480-3487 (1998)
Isolation and identification of coagulase-positive Staphylococci on Levine's eosin-methylene blue agar.
N J MENOLASINO
The Journal of laboratory and clinical medicine, 56, 908-910 (1960-12-01)

Articles

Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

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