11138600001

Interleukin-6, human (hIL-6)

recombinant (E. coli)

• Synonym(s): hIL-6, interleukin-6 human
• UNSPSC Code: 12352207

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: >98% (SDS-PAGE)
• form: solution
• potency: <0.01 ng/mL EC₅₀
• mol wt: 20,600 Da
• packaging: pkg of 200,000 U (2 μg, 1 ml)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | hybridoma: suitable
• impurities: <0.1 EU/μgtested (LAL test)
• UniProt accession no.: P05231
• storage temp.: −20°C
• Gene Information: human ... IL6(3569)

Description

General description

Interleukin-6, human (hIL-6), is produced in E. coli and purified by standard chromatographic techniques.

Specificity

Recombinant IL-6, human is effective on mouse and human cells.
IL-6, a 26 kD protein, also known as B-cell stimulatory factor 2 (BSF-2, BCDF), interferon-β₂ (IFN-β₂), hybridoma/plasmacytoma growth factor (H(P)GF), and hepatocyte-stimulating factor is produced by many cell types (e.g., T-cells, monocytes, fibroblasts, endothelial cells). IL-6 induces the terminal maturation of activated B-cells into antibody-producing cells (B-cell differentiation activity. The hepatic acute phase response is induced by IL-6 (hepatocyte-stimulating activity). It was shown that IL-6 and IL-3 act synergistically to support the proliferation of hematopoietic stem cells (hemapoietic activity), however, IL-6 does not have antiviral activity, although it has been called IFN-β₂. High affinity receptors for IL-6 are found on many cell types.

Application

Recombinant IL-6, human, is a growth factor for murine and human B-cells. It can be used to replace feeder cells in the preparation of murine and human hybridomas.

Biochem/physiol Actions

Interleukin-6 participates in several pleiotropic functions in various types of cells through its specific receptor (IL-6-R). It has been suggested that interleukin-6 may participate in the interaction between neuroendocrine and immune system by activating adrenocorticotropic hormone secretion. It has also been reported that IL-6 stimulates the bonding of receptor (IL-6-R) with a non-ligand-binding membrane glycoprotein, gp130.

Quality

Purity: 95% pure as determined by HPLC or SDS-PAGE [Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test).
Note: 1 EU corresponds to 0.1 ng

Sequence

Chain Length 184 AA

The primary structure of recombinant human IL-6 is identical to that of natural human IL-6 (one polypeptide chain, 184 amino acids), however, it contains an altered sequence within the first 15 amino-terminal amino acids.

Unit Definition

EC₅₀ definition: The amount of hIL-6 that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 7TD1 cells (1 unit equals <0.01 ng).

Physical form

Solution, filtered through 0.2 μm pore size membrane.

Preparation Note

Working concentration: 10 to 100 U/ml (0.1 1 ng/ml)
10 to 100 U/ml (0.1 to 1 ng/ml)
A concentration of 100 U/ml (1 ng/ml) is recommended for the preparation of B-cell hybridomas
Working solution: Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Reconstitution

Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.

Analysis Note

Specific activity/EC₅₀: >1 x 10⁸ U/mg, <0.01 ng/ml (hIL-6, NIBSC, interim standard, 88/514), at least the same specific activity (EC₅₀) compared to the indicated standard is guaranteed.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash