11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Synonym(s): hTNF, human necrosis factor
• UNSPSC Code: 12352207

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in yeast
• Assay: ≥95% (SDS-PAGE)
• form: solution
• potency: ≤0.01 ng/mL EC₅₀
• specific activity: >1*10^8 U/mg (>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.)
• mol wt: 17,000 Da
• packaging: pkg of 1,000,000 U (10 μg, 1ml)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.1 EU/μgtested (LAL test)
• UniProt accession no.: Q9UBM5
• storage temp.: −20°C
• Gene Information: human ... TNF(7124)

Description

General description

Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.

Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.

Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.

Specificity

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Application

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Biochem/physiol Actions

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Sequence

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Unit Definition

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Physical form

Solution, filtered through 0.2 μm pore size membrane.

Preparation Note

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash