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10378461001

Roche

Chromozym PL

90%, powder, pkg of 20 mg

Synonym(s):

chromogenic substrate

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About This Item

UNSPSC Code:
12352204

Quality Level

Assay

90%

form

powder

packaging

pkg of 20 mg

manufacturer/tradename

Roche

storage temp.

room temp

General description

Chromozym® PL is a chromogenic substrate. It is a synthetic tripeptide substrate and is more plasmin-specific.

Application

Chromozym® PL has been used:
  • to determine the plasmin enzymatic activity using human umbilical vein endothelial cells (HUVEC) cell extracts
  • as a chromogenic substrate for proteolytic activity in murine lung homogenates
  • as a substrate of plasmin in plasminogen activation assays

Quality

Purity: 90% Tosyl-Gly-Pro-Lys-4-nitranilide acetate (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Formula: C26H35N6O7SCOOCH3

Sequence

Tosyl-glycyl-polyl-lysine-4-nitranilide-acetate

Physical form

Powder

Preparation Note

Working concentration: Approximately 0.3 to 0.6 mM
Storage conditions (working solution): 2 to 8 °C
A substrate solution (3 mM) is stable for at least two weeks if stored at 2 to 8 °C. Avoid contamination with germs.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Chromozym is a registered trademark of Pentapharm AG, Basel

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Justin G Mathew et al.
Applied bionics and biomechanics, 2017, 9040161-9040161 (2017-07-01)
Vitronectin is a matricellular protein that plays an important role in both coagulation and angiogenesis through its effects on cell adhesion and the plasminogen system. Vitronectin is known to bind to endothelial cells upon integrin activation. However, the effect of
L S Engel et al.
The Journal of biological chemistry, 273(27), 16792-16797 (1998-06-27)
Comparisons of virulence between a Pseudomonas parent strain and an isogenic mutant devoid of protease IV have demonstrated a significant role for this enzyme during infection. We have characterized purified Pseudomonas aeruginosa protease IV in terms of its biochemical and
C Kluft et al.
Blood, 59(6), 1169-1180 (1982-06-01)
This study concerns a case of congenital homozygous deficiency in alpha 2-antiplasmin associated with a severe hemorrhagic diathesis. Heterozygous family members also show a mild bleeding tendency. The propositus is a 17-yr-old male born of white parents and showing a
Li-Jun Ma et al.
Journal of the American Society of Nephrology : JASN, 16(4), 966-976 (2005-02-25)
The potential and possible mechanisms for regression of existing glomerulosclerosis by angiotensin II type 1 receptor antagonist (AT1RA) and/or angiotensin I converting enzyme inhibitor (ACEI) were investigated. Adult male Sprague Dawley rats underwent 5/6 nephrectomy (Nx). Glomerulosclerosis was assessed by
Jessica L Bradshaw et al.
mSphere, 3(3) (2018-05-04)
Pneumonia is a pulmonary disease affecting people of all ages and is consistently a leading cause of childhood mortality and adult hospitalizations. Streptococcus pneumoniae and Pseudomonas aeruginosa are major lung pathogens commonly associated with community-acquired and nosocomial pneumonia. Additionally, mixed

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