MECY2MAG-73KPX

MILLIPLEX^® Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73KPX

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 77-105%; standard curve range: 1-5,000 pg/mL; (IFNγ); standard curve range: 12-50,000 pg/mL; (Exodus-2/CCL21/6Ckine); standard curve range: 12-50,000 pg/mL; (IFNβ1); standard curve range: 12-50,000 pg/mL; (IL-11); standard curve range: 12-50,000 pg/mL; (IL-20); standard curve range: 12-50,000 pg/mL; (MIP-3β/CCL19); standard curve range: 2-10,000 pg/mL; (IL-16); standard curve range: 2-10,000 pg/mL; (MCP-5/CCL12); standard curve range: 2-10,000 pg/mL; (MDC/CCL22); standard curve range: 2-10,000 pg/mL; (MIP-3α/CCL20); standard curve range: 2-10,000 pg/mL; (TARC/CCL17); standard curve range: 24-100,000 pg/mL; (Fractalkine/CX3CL1); standard curve range: 24-100,000 pg/mL; (IL-17A/F); standard curve range: 5-20,000 pg/mL; (Erythropoietin (EPO))
• technique(s): multiplexing: suitable
• compatibility: configured for Premixed
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition, the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.

The MILLIPLEX^® Mouse Cytokine/Chemokine Bead Panel II Premixed 14 Plex, contains all the components necessary to measure the following 14 biomarkers using Luminex^® xMAP^® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.

Panel Type: Cytokines/Chemokines

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

• Analytes: Erythropoietin (EPO), Exodus-2, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC (CCL22), MIP-3α/CCL20, MIP-3β/CCL19, TARC/CCL17
• Recommended Sample type: serum, plasma and cell culture
• Recommended Sample dilution: 1:2 dilution of serum and plasma. Tissue culture supernatant may require a dilution with an appropriate control medium
• Assay Run Time: overnight or one day
• Research Category: Inflammation & Immunology

Packaging

Everything you need in a single kit.

Components

Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL

Mouse Cytokine/Chemokine Panel II Premixed 14-Plex Beads, 3.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.