Skip to Content
Merck
All Photos(2)

Documents

MABT913

Sigma-Aldrich

Anti-Cytokeratin 19 Antibody, clone TROMA-3

clone TROMA-3, from rat

Synonym(s):

Keratin, type I cytoskeletal 19, CK-19, Keratin-19, K19

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TROMA-3, monoclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

technique(s)

electron microscopy: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_032497

UniProt accession no.

P19001

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... KRT19(3880)
mouse ... Krt19(16669)

General description

Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.

Specificity

Clone TROMA-3 detects cytokeratin 19 in human and murine cells.

Immunogen

Purified intermediate filaments from DM1 (Trophoblatoma) cell line.

Application

Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.

Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.

Target description

~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ana Caroline Costa-da-Silva et al.
iScience, 25(1), 103592-103592 (2022-01-11)
Chronic graft-versus-host disease (cGVHD) targets include the oral mucosa and salivary glands after allogeneic hematopoietic stem cell transplant (HSCT). Without incisional biopsy, no diagnostic test exists to confirm oral cGVHD. Consequently, therapy is often withheld until severe manifestations develop. This
Rebecca Marcus et al.
Cancers, 13(22) (2021-11-28)
Intrahepatic cholangiocarcinoma (ICC) is a primary biliary malignancy that harbors a dismal prognosis. Oncogenic mutations of KRAS and loss-of-function mutations of BRCA1-associated protein 1 (BAP1) have been identified as recurrent somatic alterations in ICC. However, an autochthonous genetically engineered mouse
Romina Lasagni Vitar et al.
Stem cell reports, 17(4), 849-863 (2022-03-26)
Severe ocular surface diseases can lead to limbal stem cell deficiency (LSCD), which is accompanied by defective healing. We aimed to evaluate the role of the substance P (SP)/neurokinin-1 receptor (NK1R) pathway in corneal epithelium wound healing in a pre-clinical

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service