MABS64

Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2

clone 6E5.2, from mouse

• Synonym(s): mitogen-activated protein kinase 14, cytokine suppressive anti-inflammatory drug binding protein, stress-activated protein kinase 2A, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting pro
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6E5.2, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_001306.1
• UniProt accession no.: Q16539
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr180/pTyr182)
• Gene Information: human ... MAPK14(1432)

Description

General description

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Specificity

This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182.

Immunogen

Epitope: Phosphorylated Thr180 and Tyr182

Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182.

Application

Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells.

Research Category
Signaling

Research Sub Category
MAP Kinases

Quality

Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.

Target description

41 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Anisomycin treated and untreated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable