MABF297

Anti-RIG-I, clone 1C3 Antibody

clone 1C3, from mouse

• Synonym(s): Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1C3, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1λ
• NCBI accession no.: NP_055129
• UniProt accession no.: O95786
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DDX58(23586)

Description

General description

Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants.

Immunogen

Recombinant protein corresponding to human RIG-I.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-549 infected with SeV cell lysate (Baum, A., et al. (2011). PNAS. 108(7):16303-16308).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-459 lung carcinoma cell lysate (Nistal-Villan, E., et al. (2010). J. Biol. Chem. 285:20252-20261).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RIG-I in HeLa cells (Courtesy of Maite Sanchez).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-RIG-I, clone 1C3 Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of RIG-I.

Quality

Evaluated by Western Blotting in A-549 infected with SeV cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected RIG-1 in 10 µg of A-549 infected with SeV cell lysate.

Target description

~110 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable