MAB2605

Anti-ATG5 Antibody, clone 177.19

clone 177.19, from mouse

• Synonym(s): APG5 autophagy 5-like, APG5 autophagy 5-like (S. cerevisiae), APG5 (autophagy 5, S. cerevisiae)-like, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5 autophagy related 5-like, Apoptosis-specific protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 177.19, monoclonal
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NM_004849.2
• UniProt accession no.: Q9H1Y0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ATG5(9474)

Description

General description

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity

Specificity

Other species have not been tested.

The antibody reacts with the 50 kDa ATG5 protein.

Immunogen

Linear Peptide, aa: 8-318

Application

Immunoprecipitation: HeLa cell lysate

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Quality

Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.

Target description

50 kDa

Linkage

Replaces: MABC137

Physical form

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot and Immunoprecipitation: HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable