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Key Documents

ABN326

Sigma-Aldrich

Anti-CAPS-2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Calcium-dependent secretion activator 2, CADPS2, CAPS-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

Q86UW7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CAPS2(84698)

General description

CAPS-2 and the related protein CAPS-1 encode novel neural/endocrine-specific cytosolic and peripheral membrane proteins. Both are essential components of the synaptic vesicle priming machinery and are required for the Ca2+-regulated exocytosis of secretory vesicles; CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, causing a selective impairment of fast phasic transmitter release. Like CAPS-1, numerous isoforms of CAPS-2 are known to exist. These isoforms show distinct expression patterns in the brain. For example, CAPS2b showed high expression in the developing cerebellum. Furthermore, one version of CAPS-2 mRNA that lacks exon 3 is detected in some autistic patients, suggesting that the differential expression pattern of CAPS-2 is involved in neuronal development.

Specificity

This antibody is predicted to be specific to CAPS-2 and not recognize CAPS-1.

Immunogen

KLH-conjugated linear peptide corresponding to human CAPS-2.

Application

Anti-CAPS-2 detects levels of CAPS-2 proteins & has been published & validated for use in WB & IHC.
Western Blot Analysis: 1 µg/mL from a representative lot detected CAPS-2 in 10 µg/mL of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected CAPS-2 in rat cerebellum tissue.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CAPS-2 in 10 µg/mL of mouse brain tissue lysate.

Target description

~160 kDa observed. Uncharacterized bands may appear at ~40 kDa, ~50 kDa, and ~210 kDa in some lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Weijun Feng et al.
Nature communications, 8, 14758-14758 (2017-03-21)
Mutations in chromatin modifier genes are frequently associated with neurodevelopmental diseases. We herein demonstrate that the chromodomain helicase DNA-binding protein 7 (Chd7), frequently associated with CHARGE syndrome, is indispensable for normal cerebellar development. Genetic inactivation of Chd7 in cerebellar granule

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