AB8345
Anti-MMP-14 Antibody
serum, Chemicon®
Synonym(s):
MT1-MMP
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MMP14(4323)
Specificity
The antibody recognizes native and recombinant proteins.
Immunogen
Catalytic domain of MT1-MMP
Application
Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Immunocytochemistry: 5-10 μg / ml
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Physical form
Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.
Storage and Stability
Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.
Analysis Note
Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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In vivo effects of zoledronic acid on oral mucosal epithelial cells.
Allam, E; Allen, M; Chu, TM; Ghoneima, A; Jack Windsor, L
Oral Diseases null
Albert G Remacle et al.
Oncotarget, 8(2), 2781-2799 (2016-11-12)
The invasion-promoting MT1-MMP is a cell surface-associated collagenase with a plethora of critical cellular functions. There is a consensus that MT1-MMP is a key protease in aberrant pericellular proteolysis in migrating cancer cells and, accordingly, a promising drug target. Because
Effects of alendronate on human osteoblast-like MG63 cells and matrix metalloproteinases.
Jun Sun,Fengyu Song,Weiping Zhang,Brent E Sexton,L Jack Windsor
Archives of Oral Biology null
David Alonso-Escolano et al.
British journal of pharmacology, 141(2), 241-252 (2003-12-24)
1. Matrix metalloproteinase-2 (MMP-2) plays a role in agonist- and tumour cell-induced platelet aggregation (TCIPA). 2. MMP-2 is synthesized as a proenzyme and is activated at the cell surface by membrane type-1 matrix metalloproteinase (MT1-MMP, MMP-14). 3. The significance of
T William Axelrad et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18(3), 568-570 (2004-01-13)
Tumor-induced angiogenic responses lead to complex phenotypic changes in vascular endothelial cells, which must coordinate the expression of both proteases and protease inhibitors prior to the proliferation and invasion of surrounding stroma. Matrix metalloproteinase 2 (MMP2), which degrades Type IV
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