648315

Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade

100 mM liquid, pH 7.4, Molecular Biology Grade

• Synonym(s): Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• MDL number: MFCD00004679
• UNSPSC Code: 12161705

Properties

• product name: Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade, Widely used biological buffer.
• grade: Molecular Biology
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• concentration: 100 mM
• color: clear colorless
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• foreign activity: DNases, RNases, none detected
• shipped in: ambient
• storage temp.: 10-30°C
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Widely used biological buffer. Prepared with diethylpyrocarbonate-treated water for molecular biology applications. Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used biological buffer. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0.

Application

• Structural Characterization of Toxicologically Relevant Cd(2+)-L-Cysteine Complexes.: This study utilized Tris buffer, 100 mM, pH 7.4, for the structural analysis of cadmium-cysteine complexes, providing insights into their biochemical behavior and toxicological impacts (Gautam et al., 2023).


• Cation and buffer specific effects on the DNA-lipid interaction.: The research employed Tris buffer, 100 mM, pH 7.4, to explore the specific effects of cations and buffers on DNA-lipid interactions, highlighting its importance in biophysical and biochemical studies (Mura et al., 2023).


• Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications.: The research utilized Tris buffer, 100 mM, pH 7.4, in the preparation of cerium-containing nanoparticles, demonstrating their potential in biomedical applications without the need for drugs (Kurtuldu et al., 2021).

Warning

Toxicity: Irritant (B)

Physical form

A sterile, 100 mM solution, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable