198250

Rose bengal

certified by the Biological Stain Commission, Dye content ≥80 %

• Synonym(s): 4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt
• Empirical Formula (Hill Notation): C₂₀H₂Cl₄I₄Na₂O₅
• CAS Number: 632-69-9
• Molecular Weight: 1017.64
• Colour Index Number: 45440
• Beilstein: 3645857
• EC Number: 211-183-3
• MDL number: MFCD00005043
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851870
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 100
• form: powder
• composition: Dye content, ≥80%
• color: dark violet
• solubility: H₂O: 1 mg/mL
• λmax: 548 nm
• ε (extinction coefficient): ≥90000 at 546-550 nm at 0.008 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1c(Cl)c(Cl)c(Cl)c(Cl)c1C2=C3C=C(I)C(=O)C(I)=C3Oc4c(I)c([O-])c(I)cc24
• InChI: 1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2
• InChI key: UWBXIFCTIZXXLS-UHFFFAOYSA-L

Description

General description

Rose Bengal is the sodium salt of tetraiodotetrachlorouorescein, C₂₀H₂O₅I₄Cl₄Na₂. It is an anionic water-soluble xanthene dye.

Application

Rose bengal has several microbilogical staining applications including:

• Conn′s technique for bacteria in soil and Kreyberg′s stain for keratin and mucus
• counterstaining hematoxylin
• staining cellular inclusions, together with Bismark brown

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves