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Merck
  • Sol-gel-based SPME fiber as a reliable sampling technique for studying biogenic volatile organic compounds released from Clostridium tetani.

Sol-gel-based SPME fiber as a reliable sampling technique for studying biogenic volatile organic compounds released from Clostridium tetani.

Analytical and bioanalytical chemistry (2017-10-17)
Masoud Ghader, Nader Shokoufi, Ali Es-Haghi, Kazem Kargosha
摘要

A novel and efficient headspace solid-phase microextraction (HS-SPME) method, followed by gas chromatography mass spectrometry (GC-MS), was developed to study volatile organic compounds (VOCs) emerging from microorganisms. Two homemade SPME fibers, a semi-polar poly (dimethylsiloxane) (PDMS) fiber, and a polar polyethylene glycol (PEG) fiber, along with two commercial fibers (PDMS and PDMS/DVB) were used to collect VOCs emerging from Clostridium tetani which was cultured in different media. The adsorbed VOCs were desorbed and identified, in vitro, using GC-MS. The adsorption efficiency was improved by optimizing the time duration of adsorption and desorption. About 50 components were identified by the proposed method. The main detected compounds appeared to be sulfur containing compounds such as butanethioic acid S-methyl ester, dimethyl trisulfide, and dimethyl tetrasulfide. These volatile sulfur containing compounds are derived from amino acids containing the sulfur element, which probably coexist in the mentioned bacterium or are added to the culture media. The developed HS-SPME-GC-MS method allowed the determination of the chemical fingerprint of Clostridium tetani volatile constituents, and thus provides a new, simple, and reliable tool for studying the growth of microorganisms. Graphical abstract Investigation of biogenic VOCs released from Clostridium tetani using SPME-GC-MS.

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Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm
Supelco
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME 纤维组件聚二甲基硅氧烷 (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 23 ga
Supelco
SPME 纤维组件聚二甲基硅氧烷 (PDMS), df 100 μm(PDMS, needle size 24 ga, for use with manual holder
Supelco
SPME 纤维组件聚二甲基硅氧烷 (PDMS), df 100 μm(PDMS, for use with autosampler, needle size 24 ga
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga
Sigma-Aldrich
甲氧基三甲基硅烷, 99%
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler
Supelco
聚丙烯酸酯 (PA) 固相微萃取萃取头, df 85 μm(PA, for use with autosampler, needle size 23 ga
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with manual holder
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, StableFlex, for use with manual holder
Supelco
聚丙烯酸酯 (PA) 固相微萃取萃取头, df 85 μm(PA, for use with autosampler, needle size 24 ga
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, needle size 24 ga, for use with autosampler
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler
Supelco
SPME 纤维组件聚二甲基硅氧烷 (PDMS), df 100 μm(PDMS, for use with manual holder, needle size 23 ga
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), df 65 μm(PDMS/DVB, for use with autosampler, needle size 23 ga
Supelco
固相微萃取 StableFlex 萃取头组合套件, for use with manual holder
Supelco
Carbowax-聚乙二醇 (PEG) 固相微萃取萃取头, needle size 23 ga, df 60 μm(PEG, for use with autosampler
Supelco
固相微萃取 StableFlex 萃取头组合套件, needle size 23 ga, for use with autosampler
Supelco
SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), df 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder
Supelco
SPME固相微萃取萃取头,二甲基硅氧烷/二乙烯基苯(PDMS/DVB), fused silica fiber, df 65 μm(PDMS/DVB, for use with manual holder, needle size 23 ga
Supelco
聚丙烯酸酯 (PA) 固相微萃取萃取头, df 85 μm(PA, for use with manual holder, needle size 24 ga