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  • nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor.

nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor.

Scientific reports (2017-07-01)
N Facchinello, T Skobo, G Meneghetti, E Colletti, A Dinarello, N Tiso, R Costa, G Gioacchini, O Carnevali, F Argenton, L Colombo, L Dalla Valle
摘要

Glucocorticoids (GCs) play important roles in developmental and physiological processes through the transcriptional activity of their cognate receptor (Gr). Using CRISPR/Cas9 technology, we established a zebrafish null Gr mutant line and compared its phenotypes with wild type and a zebrafish line with partially silenced gr (gr

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牛血清白蛋白 来源于牛血清, lyophilized powder, ≥96% (agarose gel electrophoresis)
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硫酸葡聚糖 钠盐, Mr ~40,000
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Triton X-100, laboratory grade