跳转至内容
Merck

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Nature genetics (2013-07-23)
Aarti Tarkar, Niki T Loges, Christopher E Slagle, Richard Francis, Gerard W Dougherty, Joel V Tamayo, Brett Shook, Marie Cantino, Daniel Schwartz, Charlotte Jahnke, Heike Olbrich, Claudius Werner, Johanna Raidt, Petra Pennekamp, Marouan Abouhamed, Rim Hjeij, Gabriele Köhler, Matthias Griese, You Li, Kristi Lemke, Nikolas Klena, Xiaoqin Liu, George Gabriel, Kimimasa Tobita, Martine Jaspers, Lucy C Morgan, Adam J Shapiro, Stef J F Letteboer, Dorus A Mans, Johnny L Carson, Margaret W Leigh, Whitney E Wolf, Serafine Chen, Jane S Lucas, Alexandros Onoufriadis, Vincent Plagnol, Miriam Schmidts, Karsten Boldt, Ronald Roepman, Maimoona A Zariwala, Cecilia W Lo, Hannah M Mitchison, Michael R Knowles, Rebecca D Burdine, Joseph J Loturco, Heymut Omran
摘要

DYX1C1 has been associated with dyslexia and neuronal migration in the developing neocortex. Unexpectedly, we found that deleting exons 2-4 of Dyx1c1 in mice caused a phenotype resembling primary ciliary dyskinesia (PCD), a disorder characterized by chronic airway disease, laterality defects and male infertility. This phenotype was confirmed independently in mice with a Dyx1c1 c.T2A start-codon mutation recovered from an N-ethyl-N-nitrosourea (ENU) mutagenesis screen. Morpholinos targeting dyx1c1 in zebrafish also caused laterality and ciliary motility defects. In humans, we identified recessive loss-of-function DYX1C1 mutations in 12 individuals with PCD. Ultrastructural and immunofluorescence analyses of DYX1C1-mutant motile cilia in mice and humans showed disruptions of outer and inner dynein arms (ODAs and IDAs, respectively). DYX1C1 localizes to the cytoplasm of respiratory epithelial cells, its interactome is enriched for molecular chaperones, and it interacts with the cytoplasmic ODA and IDA assembly factor DNAAF2 (KTU). Thus, we propose that DYX1C1 is a newly identified dynein axonemal assembly factor (DNAAF4).

材料
货号
品牌
产品描述

Millipore
抗-FLAG® 兔抗, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
抗-GAPDH抗体,小鼠单克隆 小鼠抗, clone GAPDH-71.1, purified from hybridoma cell culture
Sigma-Aldrich
抗γ-微管蛋白抗体,小鼠单克隆 小鼠抗, clone GTU-88, ascites fluid