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Merck
  • Dihydrofolate reductase: x-ray structure of the binary complex with methotrexate.

Dihydrofolate reductase: x-ray structure of the binary complex with methotrexate.

Science (New York, N.Y.) (1977-07-29)
D A Matthews, R A Alden, J T Bolin, S T Freer, R Hamlin, N Xuong, J Kraut, M Poe, M Williams, K Hoogsteen
PMID17920
摘要

A central eight-stranded beta-pleated sheet is the main feature of the polypeptide backbone folding in dihydrofolate reductase. The innermost four strands and two bridging helices are geometrically similar to but are connected in a different way from those in the dinucleotide binding domains found in nicotinamide-adenine dinucleotide-linked dehydrogenases. Methotrexate is bound in a 15-angstrom-deep cavity with the pteridine ring buried in a primarily hydrophobic pocket, although a strong interaction occurs between the side chain of aspartic acid 27 and N(1), N(8), and the 2-amino group of methotrexate.

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Sigma-Aldrich
甲氨蝶呤 水合物, ≥99.0% (sum of enantiomers, HPLC)