- Enhancement of neuronal cell adhesion by covalent binding of poly-D-lysine.
Enhancement of neuronal cell adhesion by covalent binding of poly-D-lysine.
Journal of neuroscience methods (2011-09-13)
Yong Hee Kim, Nam Seob Baek, Young Hwan Han, Myung-Ae Chung, Sang-Don Jung
PMID21907237
摘要
We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite per neuronal cell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronal cells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronal cells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival.
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