技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] water with 0.1% formic acid; [B] methanol
gradient: held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate: 0.6 mL/min
pressure: 4105 psi (283 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 20 μL
sample: 100 μg/mL in 90:10, water:methanol
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of silymarin.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Taxifolin
Silychristin
Apigenin 7-glucoside
Silydianin
Silybin B
Isosilybin A
Isosilybin B
分析柱
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