G004415

Improved HPLC Selectivity of Discovery^® BIO Wide Pore RP Phases

application for HPLC

属性

• 技術: HPLC: suitable
• 測試參數: column: Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 μm particles vs. competitive wide pore C18 column of same dimensions (568222-U); mobile phase: [A] 0.1% TFA in 80:20 water:acetonitrile; [B] 0.1% TFA in 66:34 water:acetonitrile; ; Gradient of 0 to 100% B in 14 min. after 1 min. delay; gradient: 0 to 100% B in 14 min. after 1 min. delay; flow rate: 1 mL/min; column temp.: 30 °C; detector: UV, 220 nm; injection: 10 μL; sample: peptide mixture (Sigma P2693), ~0.25 μg each peptide in 0.1% TFA
• 適合性: application for HPLC
• 應用: life science and biopharma

说明

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany