技術
HPLC: suitable
測試參數
column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (0.1% TFA in water):(0.1% TFA in acetonitrile) 95:5; [B] (0.1% TFA in water):(0.1% TFA in acetonitrile):50:50;
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50 μL
sample: carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
適合性
application for HPLC
應用
life science and biopharma
法律資訊
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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