568223-U

Discovery^® BIO Wide Pore C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm

• 别名: Wide Pore C18 HPLC Column
• 分類程式碼代碼: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

属性

• product name: Discovery^® BIO 大孔 C18 高效液相色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• 材料: stainless steel column
• agency: suitable for USP L1
• 產品線: Discovery^®
• 特點: endcapped
• 製造商／商標名: Discovery^®
• 包裝: 1 ea of
• 標籤範圍: 9.2% Carbon loading
• 參數: 0-70 °C temperature; 400 bar pressure (5801 psi)
• 技術: HPLC: suitable; LC/MS: suitable
• 長度 × 內徑: 25 cm × 4.6 mm
• 表面積: 100 m²/g
• 表面覆盖率: 3.6 μmol/m²
• 基質: silica particle platform; fully porous particle
• 基質活性組: C18 (octadecyl) phase
• 粒徑: 5 μm
• 孔徑: 300 Å
• 工作pH值: 2-8
• 分離技術: reversed phase

说明

一般說明

RP-HPLC 上获得的多肽谱图可提供许多关于蛋白质结构、稳定性和纯度等有价值的信息。为达到预期效果，RP-HPLC 色谱柱必须能够分离样品中高百分比的多肽。多肽含量越高，获得的信息越有用。Discovery BIO 大孔 C18 柱可从酶解物中获得分离度很高的 RP-HPLC 多肽谱图。对 Discovery BIO 大孔系列产品所采用的硅胶和键合相化学性质的改进，增加了色谱柱的柱效并减少了拖尾，因而提高分离度。另一个优点是流动相中不需要 TFA 即可分析多肽，从而增强了 LC-MS 信号。

應用

• Kinetic performance comparison of superficially porous, fully porous and monolithic reversed-phase columns by gradient kinetic plots for the separation of protein biopharmaceuticals.: This study provides a detailed kinetic performance comparison of different types of reversed-phase HPLC columns, including the Discovery^® BIO Wide Pore C18 HPLC Column. The research highlights its efficiency and resolution in separating complex protein biopharmaceuticals, making it a valuable tool for analytical chemists in biopharmaceutical analysis (Jaag et al., 2022).


• RP-LC x RP-LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases.: This paper investigates the use of reverse-phase liquid chromatography (RP-LC) with both totally porous and partially porous stationary phases, including the BIO Wide Pore C18 HPLC Column, to analyze tryptic digests. The findings demonstrate its robustness and high resolution in proteomics applications, enabling precise peptide mapping and protein characterization (Mondello et al., 2010).

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法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany