推荐产品
product name
Ascentis® RP-Amide HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
材料
stainless steel column
品質等級
agency
suitable for USP L60
產品線
Ascentis®
特點
endcapped
製造商／商標名
Ascentis®
包裝
1 ea of
標籤範圍
19.5% Carbon loading
參數
≤70 °C temp. range
400 bar pressure (5801 psi)
技術
HPLC: suitable
LC/MS: suitable
長度 × 內徑
25 cm × 4.6 mm
表面積
450 m2/g
表面覆盖率
2.7 μmol/m2
雜質
<5 ppm metals
基質
fully porous particle
silica gel high purity, spherical
基質活性組
amide, alkyl phase
粒徑
5 μm
孔徑
100 Å
工作pH值範圍
2-8
應用
environmental
food and beverages
分離技術
reversed phase
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一般說明
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。
Ascentis RP-Amide 反相酰胺柱是新一代超低流失、嵌入极性基团 (EPG) 的键合相，该键合相可提供与常规C18不同的选择性，并提高极性化合物 HPLC 和 LC-MS 分析的分离度。Ascentis RP-Amide 反相酰胺柱是嵌入极性基团 HPLC 键合相的首选。
應用
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).
特點和優勢
- 对极性化合物具有良好的保留性能和峰形
- 100% 水兼容
- 超低流失，与 LC-MS 兼容
- 独特的选择性
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
相關產品
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
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