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55267-U

Supelco

HybridSPE®-Phospholipid solid phase extraction (SPE) Cartridge

Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30

别名:

HybridSPE (phospholipid and protein removal) 96-well plate, 15 mg

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About This Item

分類程式碼代碼:
41115712
NACRES:
NB.21

product name

HybridSPE ®-磷脂SPE小柱, Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30

材料

PE frit (20/40 μm)
polypropylene hardware

品質等級

100

產品線

HybridSPE®

成份

bed wt., 500 mg

包裝

pk of 30

技術

solid phase extraction (SPE): suitable

容積

6 mL

基質活性組

zirconia-based phase

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一般說明

HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台，设计用于在 LC-MS 或 LC-MS/MS 分析之前，从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白，并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上，其作为化学过滤器，专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。

HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。

應用

  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: This study presents a method incorporating HybridSPE-Phospholipid for efficient cleanup in the analysis of various substances in biological matrices, showcasing its application in environmental toxicology (Tanoue et al., 2020).
  • Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens.: Demonstrates the use of HybridSPE-Phospholipid in multi-residue mycotoxin analysis, enhancing the detection capabilities in complex sample matrices (Lauwers et al., 2019).
  • Liquid chromatography mass spectrometry determination of perfluoroalkyl acids in environmental solid extracts after phospholipid removal and on-line turbulent flow chromatography purification.: Explores the effectiveness of HybridSPE-Phospholipid in removing interfering substances from environmental samples, aiding in the accurate analysis of perfluoroalkyl substances (Mazzoni et al., 2016).
  • Development and validation of a liquid chromatography-tandem mass spectrometry method for the quantitative determination of gamithromycin in animal plasma, lung tissue and pulmonary epithelial lining fluid.: Illustrates the adaptability of HybridSPE-Phospholipid in veterinary medicine for quantifying antibiotics, ensuring precision in therapeutic monitoring (De Baere et al., 2015).

特點和優勢

  • 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
  • 通过完全除去磷脂和沉淀蛋白，减少离子抑制
  • 2-3 步通用流程
  • 方法开发最少，甚至不需要方法开发
  • 有 96 孔和 1 mL 萃取柱规格可供选择

法律資訊

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析证书(COA)

Lot/Batch Number
16630901
15982201
14981702
14981701
13694602

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Martina Rejtharová et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 34(4), 477-481 (2016-12-06)
Monitoring of steroid esters in blood serum is desirable in order to detect the possible illegal use of natural hormones as growth promoters. A method for the determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate
Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography-tandam mass spectrometry
Hongliang, J,, et al.
Journal of Chromatography. B, Biomedical Applications, 879 (22), 2162-2170 (2011)
Nora Unceta et al.
Journal of pharmaceutical and biomedical analysis, 70, 529-533 (2012-06-01)
This work proposes a liquid chromatography-electrospray ionization ion trap mass spectrometry (LC-ESI-ITMS) method, for the quantification of sildenafil (SDF), tadalafil (TDF) and vardenafil (VDF) and their metabolites N-desmethylSDF, O-desethylSDF and N-desethylVDF, preceded by a sample preparation step based on protein
C Ferreiro-Vera et al.
Journal of chromatography. A, 1240, 21-28 (2012-04-17)
Fractionation of biological fluids for proper analysis by LC-MS of low-abundance metabolites in the presence of high-abundant endogenous metabolites is of interest, particularly when the latter cause undesirable phenomena such as ionization suppression, as is the case with phospholipids (PLs).
Improved sensitivity of sedimentary phospholipid analysis resulting from a novel extract cleanup strategy
Zhu, Z., et al.
Organic Geochemistry, 65, 46-52 (2013)
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This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

Ion-Suppression & Phospholipid Contamination

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

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实验方案

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

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