生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1J11, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 79.31 kDa
observed mol wt ~82 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
基因資訊
human ... DDX4(54514)
一般說明
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1J11 is a ZooMAb® rabbit recombinant monoclonal antibody that detects DDX4. It targets an epitope within 21 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human DDX4.
應用
Quality Control Testing
Evaluated by Western Blotting in NTERA-2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DDX4 in NTERA-2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in NTERA-2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in NTERA-2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DDX4 in NTERA-2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in NTERA-2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DDX4 in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Probable ATP-dependent RNA helicase DDX4 (UniProt: Q9NQI0; also known as EC:3.6.4.13, DEAD box protein 4, Vasa homolog) is encoded by the DDX4 (also known as VASA) gene (Gene ID: 54514) in human. Probable ATP-dependent RNA helicase DDX4, belongs to the DEAD-box protein family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX4 is primarily involved in the regulation of RNA metabolism, germline formation, fertility, and piRNA-dependent retrotransposon control. It plays a critical role in processes such as RNA splicing, ribosome biogenesis, and the regulation of translation. DDX4 is localized mainly in the cytoplasm of germ cells, where it associates with germ granules such as nuage in mammals and pole plasm in Drosophila. These granules are involved in the storage and regulation of mRNAs required for germ cell development and function. Aberrant expression or function of DDX4 can lead to infertility due to defects in germ cell development. Dysregulation of DDX4 gene is associated with reproductive disorders and certain types of germ cell tumors, such as seminomas and dysgerminomas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gustafson, E. A., et al., 2010, Biochem. Biophy. Res. Commun. 395; 1-6, Xu, C., et al., 2022, Cell. Mol. Life Sci.79; 4)
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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