ZRB2006

Anti-phospho-p38 (T180/Y182) Antibody, clone 2B7 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: EC:2.7.11.24, MAP kinase 13, MAP kinase p38 delta, MAPK 13, Mitogen-activated protein kinase 13, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4
• 分類程式碼代碼: 12352203

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2B7, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 純化經由: using Protein A
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: flow cytometry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG
• 表位序列: Internal
• Protein ID登錄號: NP_002745
• UniProt登錄號: O15264
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2B7 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects p38 MAP kinase phosphorylated on threonine 180 and tyrosine 182.

免疫原

KLH-conjugated linear peptide corresponding to 14 amino acids surrounding phosphothreonine 180 and phosphotyrosine 182 of human p38 MAP kinase delta.

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells treated with Anisomycin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-p38 MAPK (Thr180/Tyr182) in lysate from overnight serum starved HEK293 cells treated with Anisomycin (5 g/mL; 30 min.)

Tested Applications

Peptide Inhibition Assay:: Target band detection in lysate from HEK293 cells treated with Anisomycin was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected phospho-p38 MAPK (Thr180/Tyr182) in one million HEK293 cells treated with Anisomycin.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-p38 MAPK (Thr180/Tyr182) in Human colon cancer tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Mitogen-activated protein kinase 13 (UniProt: O15264; also known as EC:2.7.11.24, MAP kinase 13, MAPK 13, Mitogen-activated protein kinase p38 Delta^™, MAP kinase p38 Delta^™, Stress-activated protein kinase 4) is encoded by the MAPK13 (also known as PRKM13, SAPK4) gene (Gene ID: 5603) in human. p38 is a serine/threonine kinase that is an essential component of the MAP kinase signal transduction pathway. It plays an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Four splice variants of the p38 family have been identified and are known as p38α, p38β, p38γ, and p38δ. Of these, p38α and p38β are ubiquitously expressed while p38γ and p38δ are differentially expressed depending on tissue type. p38δ expression is observed in testes, pancreas, small intestine, lung, and kidney. It is also abundant in macrophages and is also detected in neutrophils, CD4+ T-cells, and endothelial cells. It phosphorylates a broad range of downstream proteins. Its protein kinase domain is localized in amino acids 25-308. It is fully activated following Dual^® phosphorylation on threonine 180 and tyrosine 182 by MKK3, MKK4, MKK6, and MKK7. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Ozawa, S., et al. (2010). Biochem. Biophys. Res. Commun. 396(4); 1060-1064; Zarubin, T., and Han, J. (2005). Cell Res. 15(1); 11-18; Jiang, Y., et al. (1997). J. Biol. Chem. 272(48); 30122-30128).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

Affinity is a trademark of Mine Safety Appliances Co.

Delta is a trademark of Heathrow Scientific LLC

Dual is a registered trademark of Ciba-Geigy AG

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable