ZRB1994

Anti-FOXP1 Antibody, clone 1J11-H1 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Forkhead box protein P1 (UniProt: Mac-1-regulated forkhead, MFH)
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1J11-H1, recombinant monoclonal
• 描述: 1J11-H1 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 75.32 kDa; observed mol wt ~80 kDa
• 純化經由: using Protein A
• 物種活性: monkey, human
• 物種活性（以同源性預測）: rat, mouse, bovine, porcine
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_001231745
• UniProt登錄號: Q9H334
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FOXP1(27086)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1J11-H1 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Forkhead box protein P1 (FOXP1). It targets an epitope within 16 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Forkhead box protein P1 (FOXP1).

應用

Quality Control Testing

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected FOXP1 in Daudi cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected FOXP1 in COS-7 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FOXP1 in human prostate tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FOXP1 in MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound FOXP1 with a KD of 3.1 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Forkhead box protein P1 (UniProt: Mac-1-regulated forkhead, MFH) is encoded by the FOXP1 (also known as HSPC215) gene (Gene ID: 27086) in human. FOXP1 is a transcriptional repressor that can act with C-terminal binding protein 1 (CTBP1) to synergistically repress transcription. It plays an important role in the specification and differentiation of lung epithelium. It can act as a homodimer or can form heterodimers with FOXP2 and FOXP4. Its dimerization is reported to be essential for DNA-binding and its leucine zipper domain (aa 348-369) is required for dimerization and transcriptional repression. It is shown to be an essential transcriptional regulator of B-cell development and is also involved in regulating cardiac muscle cell proliferation and columnar organization of spinal motor neurons. It promotes the formation of the lateral motor neuron column and the preganglionic motor column and is required for respective appropriate motor axon projections. FOXP1 represses transcription of various pro-apoptotic genes and cooperates with NF- B-signaling in promoting B-cell expansion by inhibition of caspase-dependent apoptosis. FOXP1 is expressed in areas of the brain associated with language such as the neocortex and striatum and mutations in FOXP1 gene have been linked to autism spectrum disorder (ASD). This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sollis, E., et al. (2016). Hum. Mol. Genet. 25(3); 546-557; Sin, C., et al. (2015). J. Mol. Neurosci. 55(2); 437-448).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable