ZRB1939

Anti-IGF-2R/CD222 Antibody, clone 2O9 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Cation-independent mannose-6-phosphate receptor;CI Man-6-P receptor;CI-MPR;M6PR;300 kDa mannose 6-phosphate receptor;MPR 300;Insulin-like growth factor 2 receptor;Insulin-like growth factor II receptor;IGF-II receptor;M6P/IGF2 receptor;M6P/IGF2R;CD222
• 分類程式碼代碼: 12352203

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2O9, recombinant monoclonal
• 描述: 2O9 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 274.38 kDa; observed mol wt ~275 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_000867
• UniProt登錄號: P11717
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... IGF2R(3482)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2O9 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects IGF-2R/CD222. It targets an epitope within 20 amino acids from the C-terminal half, lumenal domain.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human IGF-2R/CD222.

應用

Quality Control Testing

Evaluated by Western Blotting in SK-MEL-28 cell lysates.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected IGF-2R/CD222 in SK-MEL-28 cell lysates.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected IGF-2R/CD222 in Jurkat and HepG2 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound IGF-2R/CD222 peptide with a KD of 3.7 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-2R/CD222 in SK-MEL-28 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected IGF-2R/CD222 in human placenta tissue sections.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected IGF-2R/CD222 in one million HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Cation-independent mannose-6-phosphate receptor (UniProt: P11717; also known as CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, MPR 300, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, IGF-II receptor, M6P/IGF2 receptor, M6P/IGF2R, CD222) is encoded by the IGF2R (also known as MPRI) gene (Gene ID: 3482) in human. IGF-2R/CD222 is a single-pass type I membrane protein found in Golgi and endosome. It is synthesized with a signal peptide (aa 1-40), which is subsequently cleaved off to produce the mature protein that contains a long lumenal domain (aa 41-2304), a transmembrane domain (aa 2305-2327), and a cytoplasmic domain (aa 2328-2491). It contains 15 mannose 6-phosphate receptor homology (MRH) domains in its extracellular region. IGF-2R/CD222 mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors (MPR) in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic pre-lysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. IGF-2R/CD222 undergoes cysteine-S-palmitoylation that promotes interaction with the retromer cargo-selective complex which mediates its retrograde trafficking to the Golgi apparatus. IGF-2R/CD222 is reported to transport Lck intracellularly and direct its distribution to CD45 at the plasma membrane. The loss of IGF-2R/CD222 results in a decrease in signal transduction and subsequently a blockade of T cell effector functions. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pfisterer, K., et al. (2014). J. Immunol.193(6); 2718-2732; McCormick, PJ., et al. (2008). Traffic. 9(11); 1984-1997).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable