推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1O12, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 22.74 kDa
observed mol wt ~18 kDa
純化經由
using Protein A
物種活性
canine, human
物種活性（以同源性預測）
monkey, feline, bovine
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CLDN1(9076)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1O12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Claudin-1. It targets an epitope within 24 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Claudin-1.
應用
Quality Control Testing
Evaluated by Western Blotting in MDCK cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-1 in MDCK cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Claudin-1 in A431, A549 cell lysates and 1:10,000 dilution detected Claudin-1 in RT4 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Claudin-1 with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in human skin tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MDCK cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Claudin-1 in MDCK cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Claudin-1 in A431, A549 cell lysates and 1:10,000 dilution detected Claudin-1 in RT4 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Claudin-1 with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in human skin tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Claudin-1 in A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Claudin-1 (UniProt: O95832; also known as Senescence-associated epithelial membrane protein) is encoded by the CLDN1 (also known as CLD1, SEMP1, UNQ481/PRO944) gene (Gene ID: 9076) in human. Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are co-expressed and interact with each other, and this determines the overall permeability. Claudin-1 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is strongly expressed in liver and kidney and its expression is also observed in heart, brain, spleen, lung, and testis. It can form homo- and heteropolymers with other claudin family members. It also directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 and residues 210 and 211 are required for this interaction. Claudin-1 is shown to be essential for preventing the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. It is required for normal water homeostasis and prevents excessive water loss through the skin. In human liver cells, claudin-1 is reported to promote invasive behavior by activating the c-Abl-PKC signaling pathway. CLDN1 knock-out mice die at the first day of birth due to increased trans-epidermal water loss and human subjects lacking claudin-1 suffer from the Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) syndrome that includes an ichthyosiform skin phenotype. Claudin-1 is also known to associate with CD81 and the claudin-1-CD81 receptor complex is reported to be essential for hepatitis C virus (HCV) entry into host cell. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bergmann, S., et al. (2020). Sci. Rep. 10; article 2024; Singh, AB., et al. (2017). Pflugers Arch. 469(1): 69-75; Harris, HJ., et al. (2010). J. Biol. Chem. 285(27); 21092-21102).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门