ZRB1427

Anti-Macrosialin/CD68 Antibody, clone 2L16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2L16, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 37.41 kDa; observed mol wt ~33 kDa
• 純化經由: using Protein A
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: ELISA: suitable; affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal
• Protein ID登錄號: NP_001242
• UniProt登錄號: P34810
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2L16 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects human Macrosialin/CD68. It targets an epitope within 16 amino acids from the N-terminal, extracellular domain.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Macrosialin/CD68.

應用

Quality Control Testing

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Macrosialin/CD68 in U937 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound CD68 with a KD of 2.6 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis): A 1:1,000 dilution from a representative lot detected Macrosialin/CD68 in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Macrosialin/CD68 peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Macrosialin/CD68, clone 2L16 ZooMAb^®, Cat. No. ZRB1427, is a recombinant Rabbit monoclonal antibody that specifically targets of Macrosialin/CD68 and is used in Affinity Binding Assay, ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

標靶描述

Macrosialin (UniProt: P34810; also known as Gp110, CD68) is encoded by the CD68 gene (Gene ID: 968) in human. CD68 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-319), a transmembrane domain (aa 320-345), and a short cytoplasmic tail (aa 345-354). It also contains a mucin-like domain (aa 23-140) and has two 30 amino acid long repeats (aa 70-99 and 100-129), proline-rich hinge (aa 140-152), and a LAMP-like domain. CD68 is highly expressed in blood monocytes and tissue macrophages. Its expression is also observed in lymphocytes, fibroblasts, and endothelial cells. It is also found in many tumor cells and allows them to attach to selectin on vascular endothelium, facilitating their dissemination to secondary sites. CD68 is mainly located in the endosomal/lysosomal compartment but can rapidly shuttle to the cell surface. In macrophages, it plays a role in their phagocytic activities, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, which allows for homing of macrophages to particular sites. It has a good predictive value as a prognostic marker of survival in cancer subjects. CD68 expression is shown to be upregulated in macrophages in response to inflammatory stimuli, such as exposure to oxLDL, lipopolysaccharide, or interferon-g. Hence, it can serve as a good histochemical/cytochemical marker of inflammation associated with the involvement of monocytes/macrophages. Its expression is not observed in dendritic cells; hence it can be used to immunohistochemically distinguish macrophages from dendritic cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chistiakoc, DA., et al. (2017). Lab. Invest. 97(1); 4-13).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable