推荐产品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4B19, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 119.63 kDa
observed mol wt ~120 kDa
物種活性
human, mouse
物種活性（以同源性預測）
porcine
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Myo1C(17913)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 4B19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Unconventional myosin-Ic. It targets an epitope within 16 amino acids from the N-terminal region. May also display reactivity with isoform 1.
免疫原
KLH-conjugated linear peptide corresponding to the first 16 amino acids from human Unconventional myosin-Ic, isoform 3.
應用
Anti-MYO1C, clone 4B19 ZooMAb, Cat. No. ZRB1372, , a recombinant Rabbit monoclonal antibody that targets Unconventional myosin-Ic (MYO1C) and is tested in Immunocytochemistry and Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYO1C in HT29, HEK293, and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYO1C in human stomach and human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MYO1C in HeLa, A431, HUVEC, NIH 3T3, and HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYO1C in human stomach and human tonsil tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MYO1C in HeLa, A431, HUVEC, NIH 3T3, and HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Unconventional myosin-Ic (UniProt: O00159; also known as Myosin I beta, MMI-beta, MMIb, MYO1C) is encoded by the MYO1C gene (Gene ID: 4641) in human. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments. MYO1C, a single-headed class I myosin, localized to the cell surface. It is associated with cholesterol-enriched lipid rafts and enhances their recycling from intracellular compartments to the cell surface. It is involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. It is reported to colocalize with RNA polymerase II in the nucleus and with RNA polymerase I in nucleoli. In the nucleolus, it is localized predominantly in the dense fibrillar component (DFC) and in granular component. The MYO1C gene produces three alternatively spliced isoforms that differ only in their N-terminal regions. Although they share identical motor domains, three calmodulin-binding IQ motifs containing a nuclear localization signal, and a membrane-binding tail domain, each isoform has different function and nuclear cytoplasm partitioning. These isoforms display both specific and overlapping nuclear and cytoplasmic functions and have different expression levels. Isoform 3 of MYO1C is involved in regulation of transcription and associates with transcriptional active ribosomal genes and cooperates with the WICH chromatin-remodeling complex to facilitate transcription. It is shown to be essential for the formation of the first phosphodiester bond during transcription initiation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zattelman L., et al. (2017). J Biol Chem. 292(43);17804-17818).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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