推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
5E13, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 126.18 kDa
observed mol wt ~135 kDa
物種活性
human, rat
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal cytoplasmic domain
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 5E13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects potassium-chloride cotransporter 2 (KCC2). It targets at epitope within the C-terminal, cytoplasmic domain.
免疫原
His-tagged recombinant fragment corresponding to 112 amino acids from the C-terminal, cytoplasmic domain of human potassium-chloride cotransporter 2 (KCC2).
應用
Quality Control Testing
Evaluated by Western Blotting in Rat Brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected K+/Cl- Cotransporter-2 (KCC2) in Rat Brain tissue lysate.
Tested applications
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound K+/Cl- Cotransporter-2 (KCC2) recombinant protein fragment with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat Brain tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected K+/Cl- Cotransporter-2 (KCC2) in Rat Brain tissue lysate.
Tested applications
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound K+/Cl- Cotransporter-2 (KCC2) recombinant protein fragment with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in SH-SY5Y cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected K+/Cl- Cotransporter-2 (KCC2) in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-K+/Cl- Cotransporter-2, clone 5E13 ZooMAb®, Cat. No. ZRB1273, is a Rabbit monoclonal antibody that targets K+/Cl- Cotransporter-2 and is used in Affinity Binding Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting.
標靶描述
Solute carrier family 12 member 5 (UniProt: Q9H2X9; also known as Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter, hKCC2, KCC2) is encoded by the SLC12A5 (also known as KCC2, KIAA1176) gene (Gene ID: 57468) in human. KCC2 is a multi-pass homomultimeric membrane protein with seven cytoplasmic domains and twelve transmembrane domains. It can also form heteromultimers with other K+/Cl- cotransporters. Its expression is restricted to neuronal cells. KCC2 mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. It is the major extruder of intracellular chloride and establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. It is also involved in the regulation of dendritic spine formation and maturation. Overexpression of KCC2 has been reported in colorectal carcinogenesis and bladder urothelial carcinoma. Its tumor promoting effects are reported to be due to its ability to inhibit apoptosis mediated via apoptosis inducing factor-dependent and Endonuclease G-dependent apoptotic signaling pathway. Mutations in SLC12A5 gene are known to cause early infantile epileptic encephalopathy that is characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, JY., et al. (2017). Cell Death Dis. 8; e2691).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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