推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
116.04 kDa
87.06 kDa
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ITGAV(3685)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone EM22703 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Integrin αVβ3.
免疫原
Recombinant extracellular domains of Integrin alphaV (31-987) and Integrin beta3 (27-718).
應用
Anti-Integrin alphaVbeta3, clone EM22703 ZooMAb, Cat. No. ZRB1190, is a recombinant Rabbit monoclonal antibody that targets Integrin alphaVbeta3 and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a represetative lot detected Integrin alphaV beta3 in human melanoma tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a represetative lot detected Integrin alphaV beta3 in human lung cancer tissue sections.
Flow Cytometry Analysis: 1μg from a represetative lot detected Integrin alphaV beta3 in one million HUVEC.
Immunocytochemistry Analysis: A 1:100 dilution from a represetative lot detected Integrin alphaV beta3 in HUVEC and A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunofluorescence Analysis: A 1:100 dilution from a represetative lot detected Integrin alphaV beta3 in human lung cancer tissue sections.
Flow Cytometry Analysis: 1μg from a represetative lot detected Integrin alphaV beta3 in one million HUVEC.
Immunocytochemistry Analysis: A 1:100 dilution from a represetative lot detected Integrin alphaV beta3 in HUVEC and A549 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Integrin αVβ3 (UniProt: P06756/P05106; also known as CD51/CD61) is encoded by the ITGAV/ITGB3 (also known as MSK8, VNRA, VTNR/GP3A) genes (Gene ID: 3685/3690) in human. Integrin alphaV is a single-pass membrane protein that heterodimerizes with a beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular region of the alpha subunit. Alpha V associates with either beta1, beta3, beta5, beta6, or beta8 in a non-covalent manner. The structural and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. Integrin alphaV beta3 serves as a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Both Integrin alphaV and beta3 are single-pass membrane proteins that are synthesized with a signal peptide (aa 1-30 and 1-26, respectively), which is subsequently cleaved off to generate the mature form. Their mature forms contain an extracellular domain (aa 31-992/27-718), a transmembrane domain (aa 993-1016/719-741), and a cytoplasmic domain (aa 1017-1048/742-788). This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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