生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
無性繁殖
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 36.95 kDa
observed mol wt ~37 kDa
物種活性
mouse, monkey, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1G8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bmi-1. It targets an epitope within 19 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Bmi-1.
應用
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Bmi-1 in MCF-7 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Bmi-1 in K562 and Cos-7 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HeLa, A431, HepG2, and NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Bmi-1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Bmi-1 in MCF-7 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Bmi-1 in K562 and Cos-7 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HeLa, A431, HepG2, and NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Bmi-1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Bmi-1, clone 1G8 ZooMAb, Cat. No. ZRB1155, is a recombinant Rabbit monoclonal antibody that targets Bmi-1 and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
Polycomb complex protein BMI-1 (UniProt: P35226; also known as Polycomb group RING finger protein 4, RING finger protein 51) is encoded by the BMI1 (also known as PCGF4, RNF51) gene (Gene ID: 100532731) in human. BMI-I (B cell-specific Moloney murine leukemia virus integration site 1) is a component of the polycomb repressive complex 1 (PRC1) that is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. This complex acts via chromatin remodeling and modification of histones and mediates mono-ubiquitination of histone H2A lysine 119, rendering chromatin. The canonical PRC1 complex consists of four core subunits: CBX (polycomb; CBX2/4/6/7/8), PCGF (polycomb group factors; PCGF1 6), PHC (polyhomeotic homologues; PHC1/2/3), and RING E3 ligase (RING1A/B). BMI-I has three domains. The N-terminal RING domain, a central domain, and a C-terminal proline-rich domain. The RING domain forms a complex with RINGA/B proteins that constitutes the heterodimeric E3 ubiquitin ligase subunit of the PRC1 complex. The central is a ubiquitin-like (UBL) domain and is involved in protein-protein interactions and its binding partners include the polyhomeotic proteins (PHC1, PHC2, PHC3). The central domain of BMI-I is reported to be essential for its oncogenic activity and is shown to be essential for transcriptional repression activity. Higher expression of BMI1 gene is overexpressed in certain mantle cell lymphomas and several other cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gray, F., et al. (2016). Nat. Commun. 7; 13343).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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