推荐产品
生物源
mouse
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
RH95, recombinant monoclonal
monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 101.58 kDa
observed mol wt ~110 kDa
物種活性
mouse, rat
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG2a
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Gria1(14799)
rat ... Gria1(50592)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone RH95 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Glutamate receptor 1 (GluR1). It targets an epitope within the N-terminal region.
免疫原
Thyroglobulin-conjugated linear peptide from the N-terminal region of rat Glutamate receptor 1.
應用
Anti-GluR1, clone RH95 ZooMAb, Cat. No. ZMS1007, is a recombinant Mouse monoclonal antibody that specifically targets GluR1 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected GluR1 in Mouse Brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR1 in rat E18 cortical cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected GluR1 in rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR1 in rat E18 cortical cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Glutamate receptor 1 (UniProt: P19490; also known as GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic, AMPA 1, GluA1) is encoded by the Gria1 (also known as Glur1) gene (Gene ID: 50592) in rat. Four different AMPA receptors have been described and are designated as GluR1, GluR2, GluR3, and GluR4. These receptors mediate fast excitatory neurotransmission in the brain and are involved in multiple brain functions, including cognition, movement, learning, and memory. Each of the AMPA receptor subunit can exist in either ′flip′ or ′flop′ form and the efficacy of glutamate is believed to be higher at the ′flip′ form. The binding site on this receptor is formed by the N-terminal tail and the extracellular loop between transmembrane domains three and four. Upon agonist binding, these two loops move towards each other, which opens the pore.Glu-R1 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains to extracellular domains, four transmembrane domains, and two cytoplasmic domains. It is widely expressed throughout the nervous system and can exist in homotetrameric or heterotetrameric form. Tetramers may be formed by the dimerization of dimers. GluR-1 has several phosphorylation sites on the intracellular carboxy terminus. Many of these sites play a role in receptor regulation and synaptic plasticity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, HK., et al. (2010). J. Physiol. 103(1); 479-489).
外觀
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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