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材料
colorless cap
colorless tube
conical bottom
flat cap
polypropylene
polypropylene cap
polypropylene tube
描述
Autoclavable
無菌
non-sterile
特點
RNase and DNase free
autoclavable at 121 °C
graduations: no
Push fit
包裝
pack of 250 ea
case of 4 packs
技術
PCR: suitable
容量
0.2 mL
顏色
clear
適合性
suitable for (PCR applications)
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一般說明
实验方案
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
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