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Merck

X4626

Sigma-Aldrich

XTT 钠盐

powder, BioReagent, suitable for cell culture

别名:

2,3-双(2-甲氧基-4-硝基-5-磺苯基)-2H-四氮唑-5-甲酰苯胺 内盐

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About This Item

经验公式(希尔记法):
C22H16N7NaO13S2
分子量:
673.52
MDL號碼:
分類程式碼代碼:
12352207
PubChem物質ID:
NACRES:
NA.75

產品線

BioReagent

品質等級

化驗

≥90%

形狀

powder

儲存條件

dry at room temperature

技術

cell culture | mammalian: suitable
protein quantification: suitable

應用

detection

檢測方法

colorimetric

儲存溫度

2-8°C

SMILES 字串

[Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O

InChI

1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1

InChI 密鑰

JACYMBNQPPWQML-UHFFFAOYSA-M

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一般說明

XTT(3′-[1-[(苯胺基)-羰基]-3,4-四唑]-双(4-甲氧基-6-硝基)苯-磺酸钠)是一种四唑盐,在生物还原时可生成水溶性甲臜。

應用

XTT钠盐已用于评估细胞活性。
XTT可与吩嗪硫酸甲酯(PMS)联合使用,用于筛选人肿瘤细胞系。 四唑染料也被用于真菌细胞损伤研究 、葡萄球菌的抗菌敏感性测试,以及念珠菌生物膜研究。XTT检测法的局限性也有报道。

生化/生理作用

XTT(3′-[1-[(苯胺基)-羰基]-3,4-四唑]-双(4-甲氧基-6-硝基)苯-磺酸钠)受到代谢活性细胞中存在的脱氢酶的作用。这可产生水溶性甲臜,是一种颜色很深的产物。因为产物是可溶的,不会形成甲臜结晶,这一点与MTT相反,因此XTT在细胞增殖比色法检测中更具优势。

原則

在活细胞中,XTT被代谢还原,生成可比色的水溶性甲臜产物。

相關產品

产品编号
说明
价格

象形圖

Flame

訊號詞

Danger

危險聲明

危險分類

Self-react. C

安全危害

儲存類別代碼

4.1A - Other explosive hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Innovative method for quantification of cell-cell adhesion in 96-well plates
Zepeda-Moreno A, et al.
Cell adhesion & migration, 5(3), 215-219 (2011)
L M Jost et al.
Journal of immunological methods, 147(2), 153-165 (1992-03-04)
A tetrazolium compound, XTT, bioreducible to a water-soluble formazan was used to develop a simplified cellular cytotoxicity assay. Most (13/15 melanoma and 2/3 colon carcinoma cell lines tested metabolized XTT greater than 50 times more efficiently than the lymphoid effector
Amos C Hung et al.
Oncology letters, 22(5), 774-774 (2021-10-01)
Esophageal cancer is one of the most common malignancies and leading cause of cancer-associated mortality worldwide. However, the molecular mechanisms underlying esophageal cancer progression and the development of clinical tools for effective diagnosis remain unclear. Resistin, which was originally identified
Antimicrobial activity, cytotoxicity and chemical analysis of lemongrass essential oil (Cymbopogon flexuosus) and pure citral
Adukwu EC, et al.
Applied Microbiology and Biotechnology, 100(22), 9619-9627 (2016)
Wander José da Silva et al.
Brazilian dental journal, 19(4), 364-369 (2009-01-31)
2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm

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