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一般說明
亚基分子量:约 90,770
由六个亚基组成,总分子量:约 544620
由六个亚基组成,总分子量:约 544620
洋刀豆(直生刀豆)的尿素酶是一种含有840个氨基酸残基的单链六聚体,存在于植物、细菌、真菌、藻类和土壤中。它被视为是第一种含镍的酶,也是第一种结晶化酶。尿素酶以多种异构体存在,部分包括洋刀豆尿素酶(JBURE-I)、刀豆毒素(CNTX)和JBURE-IIB。JBURE-I比其他两种异构体更加丰富。尿素酶存在成熟的种子中。
應用
来自直生刀豆(洋刀豆)的尿素酶已用于:
- 使用从左心室(LV)和室间隔组织中提取的样本测定肌酸和磷酸肌酸
- 测定稳定尿样中尿素的浓度
- 一种标准品,以测定植物提取物的尿素酶活性
生化/生理作用
在植物中,尿素酶对于自然界中氮的生物利用度/代谢和防御机制至关重要。尿素酶是一种用于催化尿素水解的金属酶,以释放生长所需氮源—氨以及二氧化碳。他们表现内部肽介导的杀虫作用和杀菌活性。
脲酶活性已显示在暴露于光后被苯并芘抑制。这种抑制作用是由于苯并芘对脲酶′巯基的作用。
單位定義
一微摩尔单位是指在pH 7.0,25℃下每分钟催化尿素释放1.0 μmole NH3所需的酶活性。它相当于1.0 I.U.或0.054 Sumner单位(在pH 7.0,20℃下,5分钟内产生1.0 mg氨氮)
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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