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形狀
liquid
用途
kit sufficient for 1,000 tests
包裝
pkg of 1 kit
儲存條件
dry at room temperature
λmax
540 nm
應用
cell analysis
detection
檢測方法
colorimetric
儲存溫度
2-8°C
一般說明
用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。
應用
基于中性红的体外毒理检测试剂盒已用于:
- 测定HB8902细胞内溶酶体酶活性
- 研究人肺泡上皮细胞的细胞活力
- 研究成纤维细胞的细胞活力
生化/生理作用
中性红(NR)是一种红色染料,通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中,用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。
訊號詞
Danger
危險聲明
危險分類
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
儲存類別代碼
3 - Flammable liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
73.4 °F
閃點(°C)
23 °C
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In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
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One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with
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The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
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