跳转至内容
Merck

TOX4

Sigma-Aldrich

体外毒理学检测试剂盒,基于中性红

别名:

NR uptake assay, Neutral red assay

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84

形狀

liquid

用途

 kit sufficient for 1,000 tests

包裝

pkg of 1 kit

儲存條件

dry at room temperature

λmax

540 nm

應用

cell analysis
detection

檢測方法

colorimetric

儲存溫度

2-8°C

一般說明

用于分光光度法活细胞测定。在540 nm波长下检测转化染料的吸光度。

應用

基于中性红的体外毒理检测试剂盒已用于
  • 测定HB8902细胞内溶酶体酶活性
  • 研究人肺泡上皮细胞的细胞活力
  • 研究成纤维细胞的细胞活力

生化/生理作用

中性红(NR)是一种红色染料,通过细胞膜主动转移在溶酶体中积累。染料被提取并根据光谱定量吸收。NR摄取试验是最常用的细胞毒性试验。它广泛应用于环境和生物医学研究中,用于估计培养中的活细胞数量。NR染料体外组织培养研究用于评估病毒细胞致病性和检测有毒化合物。

訊號詞

Danger

危險聲明

危險分類

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

73.4 °F

閃點(°C)

23 °C


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

D Triglia et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
Guillermo Repetto et al.
Nature protocols, 3(7), 1125-1131 (2008-07-05)
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
Barbara Kazuń et al.
BMC veterinary research, 16(1), 216-216 (2020-06-27)
The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology
Alessandro Di Cerbo et al.
Antibiotics (Basel, Switzerland), 10(8) (2021-08-28)
One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门