T8694

TrkB/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• MDL號碼: MFCD03458837
• 分類程式碼代碼: 51111800
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in NSO cells
• 化驗: >90% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: 0.1-0.4 μg/mL ED₅₀
• 分子量: 120-130 kDa by SDS-PAGE (reducing); calculated mol wt 71 kDa
• 包裝: pkg of 100 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: western blot: suitable
• 雜質: endotoxin, tested
• UniProt登錄號: Q16620
• 儲存溫度: −20°C
• 基因資訊: human ... NTRK2(4915)

说明

應用

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

生化／生理作用

Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

其他說明

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

外觀

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

重構

Reconstitute to 100 μg/mL in phosphate buffered saline.

分析報告

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable