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Merck

T6146

Sigma-Aldrich

台盼蓝

powder, BioReagent, suitable for cell culture

别名:

直接蓝 14

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About This Item

线性分子式:
C34H24N6O14S4Na4
CAS号:
分子量:
960.81
顏色索引編號:
23850
Beilstein:
4360496
EC號碼:
MDL號碼:
分類程式碼代碼:
12352207
PubChem物質ID:
NACRES:
NA.75

產品線

BioReagent

品質等級

形狀

powder

成份

Dye content, ~40%

包裝

glass bottle of 100 g
glass bottle of 25 g
glass bottle of 5 g

儲存條件

dry at room temperature

技術

cell culture | mammalian: suitable
tissue processing: suitable

mp

>300 °C (lit.)

溶解度

H2O: 10 mg/mL

應用

cell analysis

SMILES 字串

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI 密鑰

GLNADSQYFUSGOU-UHFFFAOYSA-J

基因資訊

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一般說明

台盼蓝染料是一种来源于甲苯胺的偶氮染料它溶于水,分子量为961 g/mol。

應用

台盼蓝已被用于:
  • 细胞活力测定
  • 以0.2%台盼蓝溶液染色测定活细胞和坏死细胞的数目。
  • 对植物组织染色以观察细胞死亡情况

生化/生理作用

台盼蓝染料可根据′细胞吸收或排除染料的能力来区分死细胞和活细胞。活细胞应具有完整的细胞膜,因此将这种染料排除在外,并且细胞质是透明的。另一方面,没有活力的细胞显示出蓝色的细胞质。

準備報告

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Carc. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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