生物源
human
重組細胞
expressed in E. coli
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
分子量
~19.5 kDa
包裝
pkg of 10 μg
技術
western blot (chemiluminescent): suitable
雜質
endotoxin, tested
NCBI登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... MMP3(4314)
一般說明
Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors.
The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues.
應用
MCP-1 has been used to induce chemotaxis in mice macrophages.
MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells.
生化／生理作用
Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy.
The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent.
外觀
Lyophilized powder.
重構
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Suppression of tumor formation in vivo by expression of the JE gene in malignant cells.
Rollins BJ and Sunday ME
Molecular and Cellular Biology, 11, 3125-3131 (1991)
Rowan Pentz et al.
Neurobiology of disease, 148, 105150-105150 (2020-11-02)
Matrix metalloproteinase-3 (MMP-3) has been associated with risk of Alzheimer's disease (AD). In this study we introduce a novel role for MMP-3 in degrading nerve growth factor (NGF) in vivo and examine its mRNA and protein expression across the continuum
Simple and sensitive electrogenerated chemiluminescence peptide-based biosensor for detection of matrix metalloproteinase 2 released from living cells
Dang Q, et al.
Analytical and Bioanalytical Chemistry, 408(25), 7067-7075 (2016)
J S Frieling et al.
Oncogene, 36(31), 4498-4507 (2017-04-04)
Parathyroid hormone-related protein (PTHrP) is a critical regulator of bone resorption and augments osteolysis in skeletal malignancies. Here we report that the mature PTHrP
Hematopoietic-Derived Galectin-3 Causes Cellular and Systemic Insulin Resistance.
Li P
Cell, 167, 973-984 (2016)
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